Expressed in E. coli. DNA sequence encoding human Vimentin (amino acid residues 1 – 466; Accession # X56134) was fused with a His tag at the N-terminus.

Human Vimentin (53.7 kDa) is class III intermediate filament protein, which belongs to the intermediate filament family. It is highly expressed in the cells of mesenchymal origin. Mesenchyme later differentiates into other types of structures and systems: blood cells, endothelial cells, smooth muscle cells, circulatory system, lymphatic system. Vimentin has been used as a specific mesenchymal marker in differential diagnosis of undifferentiated neoplasms. Vimentin is widely distributed in the cytoplasm and is phosphorylated by several protein kinases in vitro.