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Native human Prostate-specific antigen / PSA Source:
Prostate-specific antigen (PSA) was purified from human seminal
plasma by ion-exchange chromatography and gel filtration. The
purified antigen was dialyzed against d.H2O and
lyophilized.
Description:
Prostate-specific antigen (PSA) is a glycoprotein of approx. 30
kDa found mainly in prostatic tissue and seminal fluid; it is
produced by normal prostate cells. The molecule consists of
approximately 92% protein and 8% carbohydrate. This enzyme
participates in the dissolution of the seminal fluid coagulum
and plays an important role in fertility. The highest amounts of
PSA are found in the seminal fluid; some PSA escapes the
prostate and can be found in the serum. This serum component has
been used to track the response to therapy in men with prostate
cancer.
Example Data:
Fig. 1. Western Blotting
Fig.1. Western Blotting analysis of native human Prostate-specific antigen (cat. no. NL-008-C100). Reducing conditions. Lane 1-3: immunostaining of PSA antigen (1 mg/ml) with anti-human Prostate-specific antigen antibody (A67-B/E3; EXBIO cat. no. 11-289-C100; dilution 1 μg/ml (1), 0.5 μg/ml (2), 0.2 μg/ml (3)). Detection by Goat anti-mouse / HRP secondary antibody. _________________________________________________________________________________________________________________________
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