NB4025
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Blocking Reagent－NB4025 ［especially human IgG in sera］
Synthetic polymer type blocking reagent.

Background:

This reagents for assay consist mainly of synthetic polymers and contain no materials derived from animals, so there is no danger of infection (such as Creutzfeldt-Jacob disease) from animals. Since synthetic polymers are the main components, all lots are quite uniform, and they can be boiled and autoclaved to sterilize and deactivate DNase. This reagents for assey are also highly stable against freezing, agitation, etc.

Feature and Advantages:

  • Blocking Reagent for Immunoassay

  • Do not contain any bio-derived materials

  • Excellent effect to non-specific binding
    -especially human IgG in sera-

Usage

  • Use it like usual blocking reagents

  • Use from 1 to 25-fold dilution
    -recommend 5-fold dilution by saline-

  • Recommend dry-treatment after blocking #Precautions for use The product contain 0.09% sodium azide as a preservatives. Be careful of intake by mistake or waste.

Characteristics:

Suppression effect for non-specific binding:

(Methods) 　After blocking a immuno-plate(5-fold dilution by saline, at 37 degree C for 2-hrs) , human sera sample(20-fold dilution by PBS) was added. After incubation for 2-hrs at 37 degree C, the plate was washed by 0.05% tween20-PBS. Then POD-labeled antibodies were added and allowed it to react 37 degree C for 2-hrs. After washing, coloring reaction (TMBZ) was conducted. Non-specific binding substances of sera was analyzed.

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Blocking Reagent NB4025 NOF-51005013 250 ML €193