TABLE OF CONTENTS

Each Instrument is completely tested and guaranteed for twelve months from delivery

The warranty applies to all the mechanical and electrical parts. It is valid only for proper

installation, use and maintenance in compliance with the instructions given in this manual.

apDia will, at its discretion repair or replace parts, which may be found defective in the warranty

period. The warranty does not include any responsibility for direct or indirect personal and material

damages, caused by improper use or maintenance of the instrument.

Parts that are inherently subject to deterioration are excluded from the warranty. In case of defects

due to misuse of the instrument, any travel and man-hour expenses will be charged extra.

In case of tenders warranty would be as per tender terms and conditions.

apDia is always accessible to the customers for any kind of information about installation, use,

maintenance and others. When asking for service, please refer to this manual and report the data

reported on the identification label (serial number).

Only qualified technicians are entitled to repair instruments.

The user should carry out ordinary maintenance.

The technical service of apDia or an authorized service center with specialized technicians, with

suitable instrumentation and original spare parts only, is always available for extraordinary

maintenance (repair), under a yearly maintenance contract or on specific demand.

This is a symbol of generic danger. It means that, serious damage can occur to the

operator if described precautions are not observed.

This is a symbol of HIGH ELECTRIC VOLTAGE. It is dangerous to touch any part

having this label. Only qualified operators can access these components, after

unplugging the instrument from the supply.

This symbol indicates that the instrument makes use of chemical reagents and other

dangerous (Corrosive, irritant, or harmful) CHEMICAL SUBSTANCES, which can

cause damage to people or materials. When this label is found, pay attention to the

This symbol indicates that the instrument involves the handling of samples, which

can be infected (urine or human serum). In this condition, infection or

contamination might occur. Pay attention to the general safety warnings when in

presence of such biological substances. Use protective clothes, gloves and glasses.

This symbol in the user manual indicates that damages to the instrument or erroneous

results could occur if the given warnings are not respected.

This symbol indicates a portion, which is particularly important, and should be

studied carefully.

This symbol indicates a Protective Earth or Ground terminal.

The instrument has to be used for the designed purposes under specified conditions, following

proper procedures and safety rules by qualified personnel.

This manual contains instructions for operation performed by qualified personnel.

A qualified user has to make sure that environmental condition is suitable, the installation is

correct, the use and maintenance are proper according to the general safety rules as well as to the

particular precautions described in the manual. (However, he is not entitled to repair the

instrument).

A qualified technician is entitled to maintain and repair the instrument using the original spare

parts according to the given instructions.

Maintain room temperature and humidity as specified in the manual.

If the instrument is not used as described in the manual, the protection provided by the instrument

may be impaired.

Alterations to the instrument are prohibited. The user is liable for any improper modification to the

instrument, and for the deriving consequences.

Contact the MANUFACTURER service or authorized service center in case the instrument need

extraordinary maintenance. Specialized technicians who will be able to repair the instrument using

original spare parts will carry out the maintenance.

This IVD equipment complies with the emission and immunity requirements as per IEC61326

series.

Warning : This equipment has been designed and tested to CISPER11 Class A. In a

domestic environment it may cause radio interference, in which case, you may need to take

measures to mitigate the interference."

An advisory that the electromagnetic environment should be evaluated prior to operation of

the device.

Warning : Do not use this device in close proximity to sources of strong electromagnetic

radiation (e.g. unshielded international RF sources), as these may interfere with the proper

operation.

A. D. TOUCH is a user-friendly micro plate Analyzer. It is compact & lightweight. It is designed

to measure and interpret enzyme immunoassay results, both monochromatically and

bichromatically. It is intended for in vitro diagnostic use.

The A. D. TOUCH can accommodate a flat bottom as well as a round configuration. The carriage

is designed in a way that the plate automatically moves smoothly and positions itself accurately in

the optical measurement path. Readings are taken continuously. The average value is calculated

and results are presented according to the option selected.

The A. D. TOUCH operates on a WIDE voltage (90-270 volt). This eliminates the need for an

The A. D. TOUCH has a special provision, which allows it to be used even when a printer is not

available. Readings can be conveniently noted down manually, by using the PAUSE option

(Ref. 8.4)

The other special features of A. D. TOUCH are the following:

result.

enzyme immunoassays. These are menu driven modes for simple and error free

operations.

Human Machine Interface TOUCH PANEL / KEYPAD

Linear measurement range 0.000 to 3.000 Absorbance Units (A).

Photometric Accuracy ± 2% or 0.007 whichever is higher, from 0 to 1.5

A

± 3% from 1.5 A to 3.0 A

Drift <0.005 A/hr

Photometric Linearity 2.5 A

Optical measurement 8 Channel

Type of filter

Wave Length

Half Bandwidth

Selection

Narrow band Interference

405nm, 450nm, 492nm, 630nm, & Two Optional

10nm ± 2nm

Automatic by Stepper Motor

Light Source

Display

Curve Plotting

Tungsten halogen lamp, 20 Watts

6” Graphics LCD, Negative Blue, STN

Graphical Representation on Printer

Plate Carrier Movement Precisely through the stepper motor

Printer

Memory

Built in Thermal Printer 52 columns

64 KB Non Volatile RAM Battery

backup supporting

125 open channels

Connectivity / RS232 Serial Port/USB 9600 baud, 8 data, 1 stop, no parity bits / USB

Analysis Mode Absorbance

Cut-off

Multi-Standard

% Absorbance

Difference

Uptake

Kinetic

Wattage

Voltage

75 Watts

115-230 Volt ± 10%, 50/60 Hz

Operation Position On horizontal flat, rigid and vibration free surface

Temperature

Relative Humidity

From + 18°C to 35°C

Up to 80%

Temperature

Relative Humidity

From -10°C to 40°C

Up to 80%

Enclosure ABS Fire Retardant

Size (cm) 36 x 36 x 22 (lxbxh)

Weight (Approx) 10 Kg

Instrument has to be decontaminated before packing for transportation.

Packaging is needed whenever the instrument is to be transported or shipped by courier or other

purposes.

To pack the instrument the following instructions has to be followed:

manual

protected by plastic protective material. Put copy of Safety clearance certificate

(copy of Safety clearance certificate is attached at the end of this manual)

The transportation of the instrument in unpacked condition must be limited within the room where

it is used, to avoid damage.

Before storing the instrument for a long period, pack it carefully as described above and store

indoors.

Relative humidity has to be less than 85%, and temperature between -10ºC and 40ºC.

the optical path. The measurement is carried out step by step.

the RS232 serial port output.

On starting the test, the plate will be conveyed into the instrument.

Plate is moved in step by step by a well-controlled mechanism and each well is positioned

precisely below the optical path. Optical density of each well is measured as explained below.

Based on selected programming mode, sophisticated algorithms are used for analyzing the

measured value and presenting the same on the printer, display & RS232 serial port for computer

Interface.

The following diagram represents the main functional elements of the instrument:

White light produced by the lamps is focused into a beam by the lens and passes through the

sample. Part of the light is absorbed by the sample and the remaining light is transmitted. It is

filtered by interference filters and focused onto the photodiodes. The photodiode converts the

received light into an electrical signal, which is transformed into a digital form, from which the

microprocessor calculates the absorbance, taking in account of the blank and bichromatic selection.

Amplifier

Analogue to Digital

Converter

Micro Processor

Key Pad Touch Panel Printer RS232 Display

Lamp

Lens

Lens

Lens

Photo sensor

Memory

Filter

Wells

1) Contrast Knob-use to adjust display contrast

2) Serial RS232 output

3) Cooling Fan

4) USB output

5) ON/OFF switch

6) SMPS

7) External printer connection

1

2

3

4

5

6

7

1) ‘YES’ key is used to select any ‘YES’ option on screen directly

2) ‘NO’ key is used to select any ‘NO’option on screen directly

3) ‘PRINT’ key is used to get a printout of current screen displayed

4) ‘FEED’ key is used to get a printout of current screen displayed

5) Navigation keys are used to select any option available on current screen

6) ‘ENTER’ key is used to run any option selected by navigation keys

7) ‘ESC’ key is used for escaping from any screen

8) ‘HOME’ key is used to bring plate carrier at home position. But this key is not active.

This function will not work.

Check accessories as per packing list.

Kindly store all packaging materials so as to use it to repack and ship for maintenance or servicing.

The instrument has to be placed on a level bench.

Room temperature has to be between 10 to 35ºC with a relative humidity below 85%.

Protect the instrument from direct sunshine.

Plug the instrument into a power source by the locally available approved plug in cable.

Power cord should be CE, CSA, and UL marked.

Please make sure that electrical power source is properly grounded.

A. D. TOUCH ELISA Analyzer is equipped with a built in 52 columns easy load thermal printer.

Procedure to load the paper is as follows:

Slide the LEVER towards back of the printer to open lock of the Paper Cover.

Lightly push up on the front of the paper cover (marked) with your thumb and rotate it towards the

back of the printer.

Close the paper cover and push down till you get locking sound.

Open the paper cover.

Remove the paper roller from the slot and place the paper roll over it as shown.

Place back the paper roller with paper into the slot provided and close the paper cover as described

before.

Keep pressing the paper feed switch until the paper feeds straight and smoothly.

HINT:

When the paper is set correctly and when the closing of the paper cover is proper the FEED LED

will not glow.

It keeps on flashing when the data is being printed.

When the paper is almost finished, red lines appear on both sides of the paper.

User can select a saved test by first touching the required “TEST” “NAME” on the screen

and then touch the “SEL“ option to carry out further operation on the test.

“DEL”: Deletes the selected test.

If the number of tests is more than 10, “NEXT” and “PREV” can be used for browsing

through the list pages

“PRINT”: For printing the test screen.

Switch on the instrument.

The instrument initializes all the parameters internally and carries out a power on self-test and

displays the following :“Please wait system initializing.”

The instrument will print the model name “A.D. TOUCH”, VERSION NUMBER, CLINIC NAME

CURRENT DATE WITH TIME.

A. D. TOUCH

apDia,

Advanced Practical Diagnostics n.v.

Hertoginstraat 82

2300 Turnhout

Belgium

A. D. TOUCH Date

Clinic name / Serial Number

MENU

Select Test SEL

1. HBSAg 6. HIV

PRNT

2. HCVAb 7. CRP

The Menus are displayed and the text of the parameter forms the TOUCH ZONE.

Above is the generic representation of a Test Screen. The Highlighted zones are TOUCH ZONES,

which are active. On touching the “Touch Zone” of a parameter, a sub menu/menu is displayed or

the requested action is carried out. The rest of the “Touch Zone” is deactivated.

For Example: To activate the selection:

Enter the primary filter value to touch any point in the shaded area “Pri” on the LCD screen.

On proper selection the analyzer responds with blinking of the parameter text and also the TOUCH

ZONE and a submenu is displayed.

To enter Test Name: Touching the “Name” touch zone provides an alphanumeric screen.

Enter the Test name by touching the Touch zone of that variable. The selected value blinks and is

displayed next to the parameter.

Selection Indicator

Selected onscreen item is shown in a shaded background. When the screen first displays, the

default selection is shown. Pressing a selection either highlights that item or activates it.

Name: POS- CutOff

Pri: 405 Sec: 0 ESC

EDIT

No Factor QC QCVal Mes. Rem RUN

BL 0 N

NC 0 1.000 N

PC 0 1.000 N

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.000

CUTABS:

QC1 N Interpretation :

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

The instrument is provided with a micro titer plate carriage to move the micro titer plate inside.

This carriage is driven by stepper motor with timing belt. It places each well of plate exactly below

the optical path of each channel. The plate carrier is moved by a well-controlled stepper motor

drive.

Maximum number of Blanks = 5

Maximum number of Controls:

1. Negative Control = 05

2. Positive Control = 05

3. Cut of control = 05

4. Low Positive Control = 05

Total number of 20 controls can be run in any combination.

Pipetting sequence should be as shown below.

Checking of readings should be done through controls reading within range specified in data sheet

of controls (care should be taken while preparing and pipetting controls and reagents, reagent and

control expiry dates need to be checked).

Do not use any sharp objects on the Touch Screen. Always use the STYLUS provided to

operate the touch panel.

Always check for proper grounding before installation. Never operate the instrument when

ground wire is removed.

Do not attempt to open the instrument and make repairs without proper technical

training. Do not allow unauthorized persons to operate or repair the instrument.

Use a clean plate and follow the instructions for blanking and standardizing. Do not

read any wells containing bubbles or dust particles.

The volume of sample, calibrators and blanks should be identical for correct readings.

The absorbance is proportionate to the path length. Pipetting should also be proper.

Monitoring of the printed values or displayed values during operation may help detect an

error.

Check the linearity and calibration of the instrument regularly against some standard

reference.

Check the micro wells before use. They should be scratch-free. The micro well track in

protected from scratches.

Recheck the reading of high OD (above 2A).

Place Plate carefully on the tray.

Ensure that the main power switch is in OFF position before connecting.

Plug the instrument to the AC mains. Confirm proper grounding for trouble free operation.

In this mode the instrument gives only absorbance of all wells of plates which containing control,

calibrators and samples.

MAIN MENU

1 Test Operations ESC

2 Technical Diagnosis

3 Utilities

4 View Plate

5 Serial Communication

TEST OPERATIONS

1 Select Test By Name

ESC

2 Select Test by Mode

3 Select Test by Number

4 List Tests

5 Add New Test

Select Test Mode

1> Absorbance 0

2> Single Standard 0

3> Cut off 0

4> Multi Standard 0

5> Percent Absorbance 0

6> Uptake 0

7> Kinetic 0

NORM – ABSORBANCE

Name : BL N ESC

Pri : 405 Sec : 0

NORM

MODE

DIFF

NORM – ABSORBANCE

Name:

ABS 450

A B C D E F G H

I J K L M N O P ENTER

Q R S T U V W X CLEAR

Y Z , - + _ 1 2

3 4 5 6 7 8 9 0

NORM – ABSORBANCE

Name : ABSORBANCE

BL Y ESC

Pri : 405 Sec : 0

NORM

MODE

DIFF

If you select “BL Y”, it will read

first well first strip ‘A1’ as a

blank and subtract the absorbance

of blank well from all other

well’s absorbance.

Pri: 450 ESC

Sec: 630

Select Filter Val:

0 405 450

492 630 XXX

YYY

Normal Absorbance Mode: Instrument gives the absorbance of each and every well.

Select touch zone “NORM”.

Differential Absorbance Mode: Instrument shows the difference of absorbance between odd

number wells and even number wells. For Example,- Strip 1,- Strip 2, - Strip 3, - Strip 4, - Strip 5, -

Strip 6. For this Mode select touch zone “DIFF”.

NORM – ABSORBANCE

Name : ABSORBANCE

BL N ESC

Pri : 405 Sec : 630

NORM

MODE

DIFF

DIFF – ABSORBANCE

Name : ABSORBANCE

BL N ESC

Pri : 405 Sec :630

NORM

MODE

DIFF

In this mode the instrument accepts the calibrator singly or in duplicate and than calculates the

concentration based on the single point standard curve passing through the point 0.0.

A single calibrator/standard of a known concentration is used to calibrate the instrument so that the

concentration of unknown samples can be calculated according to Beer’s Law. The absorbances

are read at user-selected wavelengths. If Blank is selected, instrument will automatically blank on

the first well and subtract its absorbance from each subsequent well. The second well is treated as

the calibrator/standard well. The third well is also treated as calibrator/standard well if the

calibrator/standard is in duplicate.

CALCULATION:

Sample Concentration = (Calibrator concentration /Calibrator Abs.) x Sample Abs.

Entry of all the parameter is similar to Multi standard mode (Please refer Multi standard for entry

of parameters)

Name: TSH SINGLE STANDARD

Pri: 450 Sec: 630 BL Y 0.000 CAL 1 ESC

DUP N DUP N

SAVE

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

High>

Range

Low<

In this mode cut-off point is determined for interpretation of specimens as per formula given in the

reagent manual. The negative controls are read followed by the positive controls, Cut-off control

and low positive control. Blanking on the first well is optional. The instrument calculates the

average of the negative controls and the average of the positive control, Cut-off controls and Low

positives are also calculated.

Select Test Mode

1> Absorbance 0

2> Single Standard 0

3> Cut off 0

4> Multi Standard 0

5> Percent Absorbance 0

TEST OPERATIONS ESC

1 Select Test By Name

2 Select Test by Mode

3 Select Test by Number

4 List Tests

5 Add New Test

Alphanumerical characters will appears on the screen as shown below after touching “Name” on

the screen. Enter the name of the test by selecting the individual characters one by one. After

selecting all the characters select Enter option in touch screen for confirmation of test Name.

Name: POS- CutOff

HBSAG

A B C D E F G H

I J K L M N O P ENTER

Q R S T U V W X CLEAR

Y Z , - + _ 1 2

3 4 5 6 7 8 9 0

Name: POS- CutOff

Pri: 450 Sec: 0 ESC

No

Factor QC QCVal Mes. Rem SAVE

BL 0 N

NC 0 1.000 N

PC 0 1.000 N

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.000

CUTABS:

QC1 N Interpretation :

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

Select the filter required by selecting the filter from Select filter value region after touching ‘Pri”

zone.

Name: HBSAG POS- CutOff

Pri: 450 Sec: 630 ESC

No Factor QC QCVal Mes. Rem SAVE

BL 0 N

NC 0 1.000 N

PC 0 1.000 N

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.000

CUTABS:

QC1 N Interpretation :

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

Pri: 450 ESC

Sec: 630

Select Filter Val:

0 405 450

492 630 XXX

YYY

Name: HBSAG POS- CutOff

Pri: 450 Sec: 630 ESC

No Factor QC QCVal Mes. Rem SAVE

BL 0 N

NC 0 1.000 N

PC 0 1.000 N

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.000

CUTABS:

QC1 N Interpretation :

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

Name: HBSAG POS- CutOff

Pri: 450 Sec: 630 ESC

No Factor QC QCVal Mes. Rem SAVE

BL 0 Y 0.000 0.000

NC 0 1.000 Y 0.000 0.000

PC 0 1.000 Y 0.000 0.000

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.000

CUTABS:

QC1 N Interpretation :

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

No. of Blanks = 1 ESC

Values Of BL <= 0.000

Numeric Value

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

No. of Blanks = 1.000 ESC

Values Of BL <= 0.100

Numeric Value

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

Name: HBSAG POS- CutOff

Pri: 450 Sec: 630 ESC

No Factor QC QCVal Mes. Rem SAVE

BL 1 Y 0.100

NC 0 1.000 Y

PC 0 1.000 Y

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.000

CUTABS:

QC1 N Interpretation:

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

No. of NC’s = 3 ESC

Values Of X = 1.000

Value of NC <= 0.150

Numeric Value

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

Name: HBSAG POS- CutOff

Pri: 450 Sec: 630 ESC

No

Factor QC QCVal Mes. Rem SAVE

BL 1 Y 0.100

NC 3 1.000 Y 0.150

PC 0 1.000 Y

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.000

CUTABS:

QC1 N Interpretation :

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

Name: HBSAG POS- CutOff

Pri: 450 Sec: 630 ESC

No

Factor QC QCVal Mes. Rem SAVE

BL 1 Y 0.100

NC 3 1.000 Y 0.150

PC 0 1.000 Y

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.000

CUTABS:

QC1 N Interpretation:

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

No. of PC’s = 2 ESC

Values Of Y= 1.000

Value of PC >= 0.500

Numeric Value

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

Name: HBSAG POS- CutOff

Pri: 450 Sec: 630 ESC

No

Factor QC QCVal Mes. Rem SAVE

BL 1 Y 0.100

NC 3 1.000 Y 0.150

PC 2 1.000 Y 0.500

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.000

CUTABS:

QC1 N Interpretation :

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

After selecting the “CUTFAC” following screen will come on the display:

Name: HBSAG POS- CutOff

Pri: 450 Sec: 630 ESC

No Factor QC QCVal Mes. Rem SAVE

BL 1 Y 0.100

NC 3 1.000 Y 0.150

PC 2 1.000 Y 0.500

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.000

CUTABS:

QC1 N Interpretation:

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

Value of Cut Factor = 0.200 ESC

Numeric Value

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

After selecting the “CUTABS” below screen will come on the display.

NC = Mean of NCs * Factor X

PC = Mean of PCs * Factor Y

CUTABS:

NC PC LC CC CF ENTER

+ - , * % CLEAR

1 2 3 4 5 6

7 8 9 0 .

( )

Sqrt Lg ALg Ln ALn Abs

Name: HBSAG POS- CutOff

Pri: 450 Sec: 630 ESC

No Factor QC QCVal Mes. Rem SAVE

BL 1 Y 0.100

NC 3 1.000 Y 0.150

PC 2 1.000 Y 0.500

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.200

CUTABS:

QC1 N Interpretation :

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

LC = Mean of LCs * Factor W

CC = Mean of CCs * Factor Z

CF = Cut off Factor

Sqrt = Square root

Lg = LOG ALg = Anti LOG

Ln = Natural LOG Aln = Anti Natural LOG

Abs. = any absolute value

For equation CUT OFF ABS = (0.45 * NC) + (0.35 * PC) + 0.10, the formula can be entered in

two ways.

1. Enter the values as it is i.e. CUTABS = (0.45*NC) + (0.35*PC) + 0.010. Keep the multiplication

factors X, Y, W, Z of controls NC, PC, CC and LC a constant number ‘01’. (Numbers,

mathematical functions and symbols for NC, PC, CC and LPC can be selected from the screen).

2. In second method the formula can be entered as CUT ABS = NC + PC + CF. (Since the Value of

X, Y, Z, W is entered in the QC option of NC, PC, CC, LPC and Cut off factor in the CUT FAC

option, there is no need to enter the same data again). Means here Multiplication Factor for NC = X

= 0.45

Multiplication factor for PC = Y = 0.35 and the value of cut of factor = CUT FAC = 0.100.

This is because here NC = Mean of NCs * Factor X, PC = Mean of PCs * Factor Y as mentioned

above.

If ‘CUTOFF ABS’ = (NC + PC) / 6. This can be entered as it is CUTABS = (NC + PC) / 6 by

keeping the multiplication factors X, Y, W, Z of controls NC, PC, CC and LC a constant number

‘01’.

As you know in mathematics (NC + PC) / 6 = (NC / 6) + (PC / 6) =

(1/6) * NC + (1/6) * PC = (0.166 * NC) + (0.166 * PC),

So, you can enter same equation as CUT ABS = NC + PC, by entering

Multiplication Factor for NC = X = 0.166, multiplication factor for PC = Y = 0.166.

This is because here NC = Mean of NCs * Factor X, PC = Mean of PCs * Factor Y as mention

above.

For example some kit may give QC check equation like| PCx – NCx |> 0.500,

PC / NC > 15, etc. This equation can be entered by selecting these QC options. Select touch zone

‘N’.

You will obtain ‘Y’, as shown in screen below by selecting touch zone ‘QC1’ and entering QC

equation.

Enter equation of QC and value of QC check by selecting touch zone ‘QC1 Value’ and ‘QC1

Name: HBSAG POS- CutOff

Pri: 450 Sec: 630 ESC

No Factor QC QCVal Mes. Rem SAVE

BL 1 Y 0.100

NC 3 1.000 Y 0.150

PC 2 1.000 Y 0.500

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.200

QC1 > Y Interpretation :

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

Same way you can enter QC equations for QC2, QC3 and QC4.

On screen it is mentioned that “QC1 > Y”. In most of the reagent inserts it is mentioned that QC

value should be greater than some constant value. Sometimes in the box insert it is mentioned that

the value should be less than some constant value. In such case, you can change the symbol from

“QC1 > Y” to “QC1 < Y”, only by selecting a symbol of comparison.

If any QC check is selected to ‘YES’ option, the system will not save the test without entering the

QC equation and QC check value of equation.

Enter QC Value and Equation:

QC1 Value: 0.500 ESC

QC1 Equat :

Numeric Value

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

Enter QC Value and Equation:

QC1 Value: 0.000 ESC

QC1 Equat

QC Equat: PC - NC

NC PC LC CC SA ENTER

+ - , * % CLEAR

1 2 3 4 5 6

7 8 9 0 .

( )

Sqrt Log ALog Ln ALn Abs

Enter RANGE for interpretation of results either in percentage (Greyzone) or positive and negative

values of INDEX, as a ratio of (Sample Abs. / Cutoff Abs.). If Cutoff Index range or Greyzone is

not mentioned do not enter any values. Instrument will do the interpretation with references to the

Cut off absorbance.

Name: HBSAG POS- CutOff

Pri: 450 Sec: 630 ESC

No

Factor QC QCVal Mes. Rem SAVE

BL 1 Y 0.100

NC 3 1.000 Y 0.150

PC 2 1.000 Y 0.500

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.200

CUTABS: NC + PC + CF

QC1 > Y 0.500 Interpretation :

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

Enter % Value :

% Value = 10.00 ESC

Numeric Value

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

Enter Greyzone percentage value

If you enter ‘Greyzone’ percentage

‘10.00’, instrument will show you

the same as shown in screen. It

makes the ‘Cutoff Index Range’

option ‘NO’. This means when you

are using a ‘Greyzone’ option the

‘Cut off Index Range’ option will

not work.

It is also provided to do the interpretation using ‘Cutoff Index’ by entering normal range for

‘Positive’ and ‘Negative’.

Name: CPC POS- CutOff

Pri: 450 Sec: 630 ESC

No Factor QC QCVal Mes. Rem SAVE

BL 1 Y 0.100

NC 3 1.000 Y 0.150

PC 2 1.000 Y 0.500

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.200

CUTABS: NC + PC + CF

QC1 > Y 0.500 Interpretation :

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

Name: CPC POS- CutOff

Pri: 450 Sec: 630 ESC

No

Factor QC QCVal Mes. Rem SAVE

BL 1 Y 0.100

NC 3 1.000 Y 0.150

PC 2 1.000 Y 0.500

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.200

CUTABS: NC + PC + CF

QC1 > Y 0.500 Interpretation :

QC2 N Greyzone % (Y) 10.00

QC3 N Cut off Index (N)

QC4 N Range

In case of ‘Greyzone’, it gives the

‘Positive’ remarks to sample

absorbance greater than greyzone

area of cutoff absorbance and a

‘Negative’ remarks to sample

absorbances less than greyzone

area of cutoff absorbance. The

sample absorbances lying in

greyzone area get remarks

‘Equivocal’ = ‘EQ’.

Cut off Index is the ratio of

Sample Absorbance / Cut off

Absorbance.

When you select ‘Cutoff Index’ –

‘Y’, the ‘Greyzone %’ will

become ‘NO’, means inactive.

In ‘Cutoff Index’, provide the

range for interpretation, the

positive and negative value of

ratio (Sample Abs. / Cutoff Abs.)

RESULT RANGE:

POSITIVE >= 1.000 ESC

NEGATIVE <= 0.900

Numeric Value

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

In case of ‘Cutoff Index Range ’,

It gives the ‘Positive’ remarks to

sample having index value, a

ratio of …

(Sample abs. / Cutoff Abs.)

greater than or equal to entered

‘POSITIVE’ value and

it gives ‘Negative’ remarks to

sample having index value, a

ratio of

(Sample abs. / Cutoff Abs.) less

than entered ‘NEGATIVE’

value.

The sample having index value in

between ‘POSITIVE’ and

‘NEGATIVE” range get remarks

‘Equivocal’ = ‘EQ’.

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No Factor QC QCVal Mes. Rem SAVE

BL 1 Y 0.100

NC 3 1.000 Y 0.150

PC 2 1.000 Y 0.500

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.200

CUTABS: NC + PC + CF

QC1 > Y 0.500 Interpretation :

QC2 N Greyzone % (Y) 10.00

QC3 N Cut off Index (N)

QC4 N Range

Name: CPC POS- CutOff

Pri: 450 Sec: 630 ESC

No Factor QC QCVal Mes. Rem SAVE

BL 1 Y 0.100

NC 3 1.000 Y 0.150

PC 2 1.000 Y 0.500

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.200

CUTABS: NC + PC + CF

QC1 > Y 0.500 Interpretation :

For example:

If you enter POS >= 1.0 and

NEG <=0.9.

Then, the sample will get

POSITIVE remarks having

INDEX Value,

(Sam. Abs./ Cutoff Abs.) >=

1.000.

The sample will get NEGATIVE

remarks having INDEX Value,

0.900.

The sample will get

EQUIVOCAL remarks having

INDEX Value,

(Sam. Abs./ Cutoff Abs.) in the

range from 0.900 to 1.000.

For Reverse Cut Off mode, select touch zone ‘POS’- Cut off key. It will change to REV- Cut Off.

In Reverse Cut Off Mode, programming of test is the same as Positive Cut off Mode. Only QC

check conditions for blanks and controls get reversed. In the ‘INTERPRETATION’, sample

absorbance, which is lesser than cut off absorbance, gets remarked POSITIVE. Sample absorbance

higher than cut off absorbance, gets remarked NEGATIVE. This is exactly the opposite of normal

POSITIVE Cut Off Mode. Same way in case you select ‘Cutoff Index Range’.

In this mode the instrument accepts a maximum of 12 calibrators and calculates concentration

Name: REV- CutOff

Pri: 450 Sec: 0 ESC

No

Factor QC QCVal Mes. Rem SAVE

BL 0 N

NC 0 1.000 N

PC 0 1.000 N

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.000

CUTABS:

QC1 N Interpretation :

QC2 N Greyzone % (Y) 10.00

QC3 N Cut off Index (N)

QC4 N Range

Select Test Mode

1> Absorbance 0

2> Single Standard 0

3> Cut off 0

4> Multi Standard 0

5> Percent Absorbance 0

6> Uptake 0

7> Kinetic 0

TEST OPERATIONS ESC

1 Select Test By Name

2 Select Test by Mode

3 Select Test by Number

4 List Tests

5 Add New Test

After selecting the Multistandard mode below screen will come on the display

Test Name can be entered by Selecting Touch Zone “Name:”.

Procedure for entering the test name is the same as the one of cut off mode.

Refer Test Name entry in Cut off mode for further details.

Select Touch Zone “Pri:”

Name: MULTISTANDARD

Pri: 405 Sec: 0 BL N CAL 2 ESC

DUP N DUP N

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

Name: TSH MULTISTANDARD

Pri: 405 Sec: 0 BL N CAL 2 ESC

DUP N DUP N

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

Select the filters as per details given in the reagent manual by selecting the filter number given in

the “Select Filter value:” option.

Pri: 450 ESC

Sec: 630

Select Filter Val:

0 405 450

492 630 XXX

YYY

Name: MULTISTANDARD

Pri: 405 Sec: 0 BL N CAL 2 ESC

DUP N DUP N

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

Name: MULTISTANDARD

Pri: 405 Sec: 0 BL N 0.000 CAL 2 ESC

DUP N DUP N

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

Name: MULTISTANDARD

Pri: 405 Sec: 0 BL N CAL 2 ESC

DUP N DUP N

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

YES

Blank Required ?

NO

After selecting “CAL” the following screen will appear on the display:

Select Touch Zone “No. Of Calibrators”. Numerals will display on the bottom of the screen.

Select the number of calibrators (User can select min 2 and max. 12 standard in this mode).

After entering the number of calibrators, concentration and absorbance column will come on the

display as shown below.

To enter concentration values select 0.000 in Conc. column corresponding to the standard and enter

the concentration value. e.g. for entering Concentration of standard 4 Select 0.000 in fourth row of

Conc. Column and enter the concentration by selecting the numerals.

(Note: The concentration value should be either ascending or descending order.)

No Of Calibrator 2 ESC

No Conc Abs

1 0.000 0.000

2 0.000 0.000

Numeric Value

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

No Of Calibrator 7 ESC

No Conc Abs

1 0.000 0.000

2 0.500 0.000

3 1.000 0.000

4 2.500 0.000

5 5.000 0.000

6 7.500 0.000

7 15.00 0.000

Numeric Value

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

Select touch zone “DUP” below the “BLN”

Name: MULTISTANDARD

Pri: 405 Sec: 0 BL Y 0.000 CAL 7 ESC

DUP N DUP N

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

Name: TSH MULTISTANDARD

Pri: 450 Sec: 630 BL Y 0.000 CAL 7 ESC

DUP N DUP N

Graph : LINEAR SAVE

Y vs X : ABS vs CONC

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

After selecting “DUP” instrument will display message “ Duplicate Blank Yes/No” as shown

below. Select “Yes” for Duplication of Blank.

To select Duplicate Calibrator select Touch Zone “DUP” below the “CAL 7”

Name: MULTISTANDARD

Pri: 405 Sec: 0 BL Y 0.000 CAL 7 ESC

DUP N DUP N

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

YES

Duplicate Blank?

NO

Name: MULTISTANDARD

Pri: 405 Sec: 0 BL Y 0.000 CAL 7 ESC

DUP Y DUP N

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

Screen after standard entry & selection of duplicate – Blank & Calibrators.

Name: MULTISTANDARD

Pri: 405 Sec: 0 BL Y 0.000 CAL 7 ESC

DUP Y DUP N

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

YES

Duplicate Calibrator?

NO

Name: MULTISTANDARD

Pri: 405 Sec: 0 BL Y 0.000 CAL 7 ESC

DUP Y DUP Y

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

There are two types of graphs in Multistandard

Linear (LINEAR)

Point to Point (PT TO PT)

There are five types of scales for X and Y axis

LOGABS vs. CONC (X-axis = Concentration v/s Y-axis = LOG of ABS.)

ABS vs. LOGCONC (X-axis = LOG of concentration v/s Y-axis = Absorbance)

LOGITABS VS LOGCONC (X-axis = LOG of concentration v/s Y-axis = LOGIT of Absorbance)

ABS vs. CONC (X-axis = Concentration v/s Y-axis = Absorbance)

LOGABS vs. LOGCONC (X-axis = LOG of concentration v/s Y-axis = LOG of absorbance)

Selection of Graph

Select Graph type as per the details given in the reagent manual by selecting Touch Zone “Graph”.

Name: MULTISTANDARD

Pri: 405 Sec: 0 BL N CAL 2 ESC

DUP N DUP N

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

After selecting the “Graph” follow screen will come on the display.

Select Graph type as Linear.

Select scale as per the details given in the reagent manual.

Select touch zone “PT to PT” abs scale of graph ‘ABS vs. CONC’.

Graph : LINEAR ESC

Y vs X : ABS vs CONC

TYPE SCALE

LINEAR LOGABS vs CONC

ABS vs LOGCONC

PT TO PT LOGITABS vs LOGCONC

ABS vs CONC

LOGABS vs LOGCONC

Graph Type : PT TO PT ESC

Y vs X : ABS vs CONC

TYPE SCALE

LINEAR LOGABS vs CONC

ABS vs LOGCONC

PT TO PT LOGITABS vs LOGCONC

ABS vs CONC

LOGABS vs LOGCONC

For High control select zone ‘HI CO: N’ Following message appears: “High Control Required?

Yes / No”. Select ‘YES’ and then enter range of control “HIGH” and “LOW”.

Name: MULTISTANDARD

Pri: 405 Sec: 0 BL N CAL 2 ESC

DUP N DUP N

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

YES

High Control Required?

NO

Name: MULTISTANDARD

Pri: 405 Sec: 0 BL N CAL 2 ESC

DUP N DUP N

SAVE

Graph : PT TO PT

Y vs X : ABS vs CONC

HI CO : N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

Enter High Range Value: ESC

High 25.03

Low 10.54

Numeric Value

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

Name: MULTISTANDARD

Pri: 405 Sec: 0 BL N CAL 2 ESC

DUP N DUP N

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>25.03

Low<10.54

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

Enter the range ‘High’ and ‘Low’ as per the reagent manual.

Name: MULTISTANDARD

Pri: 405 Sec: 0 BL N CAL 2 ESC

DUP N DUP N

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>25.03

Low<10.54

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

YES

Low Control Required?

NO

High> 16.61 ESC

Low< 5.45

Numeric Value

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

For interpretation of specimen result (POSITIVE or NEGATIVE), enter normal range given in

REAGENT MANUAL. Select key “Interpretation: N”. It will become “Interpretation: Y”.

Then select key “HIGH>” and “LOW<” to enter normal range.

Enter Normal Range for positive and negative samples.

Name: MULTISTANDARD

Pri: 0 Sec: 0 BL N CAL 2 ESC

DUP N DUP N

SAVE

Graph : LINEAR

Y vs X : ABS vs CONC

HI CO : N High>25.03

Low<10.54

LO CO: N High>16.61

Low<5.45

Interpretation: N

Range High>

Low<

Enter HIGH Range value:

High> 15.25 ESC

Low< 9.46

Numeric Value

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

All the results whose

concentration is coming above

the High Range will be

considered as positive and

coming below the Low Range

will be consider as negative.

The results having

concentration in between High

and Low range will get remarks

Name: TSH MULTISTANDARD

Pri: 450 Sec: 630 BL Y 0.000 CAL 7 ESC

DUP N DUP N

SAVE

Graph : LINEAR

Y vs. X : ABS vs CONC

HI CO : N High>25.03

Low<10.54

LO CO: N High>16.61

Low<5.45

Interpretation: Y

Range High> 15.25

Low< 9.46

The Percentage Absorbance Mode requires one calibrator (read singly or duplicate). In this mode,

calibrator is considered to have a concentration of 100%. The absorbance’s of unknown samples

are read and compared to the calibrator absorbance, and reported as % concentration of calibrator.

In this mode the instrument accepts the calibrator singly or in duplicate and then calculates the

concentration based on the single point standard curve passing through the point 0.0.

A single calibrator/standard of a known concentration is used to calibrate the instrument so that the

concentration of unknown samples can be calculated according to Beer’s Law

Sample Concentration = Calibrator Absorbance * Calibrator Concentration

Sample Absorbance

Please refer single standard for parameter entry.

Name: TSH % ABSORBANCE

Pri: 450 Sec: 630 BL Y 0.000 CAL 1 ESC

DUP N DUP N

SAVE

HI CO: N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

Name: TSH UPTAKE

Pri: 450 Sec: 630 BL Y 0.000 CAL 1 ESC

DUP N DUP N

SAVE

HI CO: N High>

Low<

LO CO: N High>

Low<

Interpretation: N

Range High>

Low<

whole test. You need to enter it only in

minutes.

“Read Period” is time interval between

two readings. You must enter it in

seconds. The instrument will

automatically calculate the number of

readings. Select primary and secondary

filters, give a name to test and save it.

Select “RUN” to start.

KINETIC

Name : KINETIC ESC

Pri : 405 Sec : 0 SAVE

Read Time : 1 min.

Read Period : 15 sec.

KINETIC

Name : KINETIC ESC

Pri : 405 Sec : 0 EDIT

Read Time : 1 min. RUN

Read Period : 15 sec. PRINT

Name: KINETIC KINETIC

Pri : 405 Sec : 630

D/M D/M D/M D/M D/M D/M

1 2 3 4 5 6

D/M D/M D/M D/M D/M D/M

7 8 9 10 11 12

YES

Is Plate Loaded

NO

ESC PRINTMATRIX SEND PLATE LOAD NEXT

Name: KINETIC KINETIC

Pri : 405 Sec : 630

D/M D/M D/M D/M D/M D/M

1 2 3 4 5 6

D/M D/M D/M D/M D/M D/M

7 8 9 10 11 12

YES

Is Plate Removed

NO

ESC PRINTMATRIX SEND PLATE LOAD NEXT

In “PRINT MATRIX”, you will get the print of entire plate data with initial absorbance of each

sample, average delta values of each sample and “D/M” values of each well, like…

A

W1 W2 W3 W4 W5 W6

0.200 0.100 1.200 0.300 2.600 0.900.. These are initial absorbances.

0.010 0.001 0.005 0.017 0.028 0.001.. These are average delta values.

0.020 0.002 0.010 0.035 0.056 0.001.. These are “D/M” values of each sample.

B

W1 W2 W3 W4 W5 W6

0.300 0.100 1.200 0.200 2.600 0.800..

0.015 0.028 0.037 0.050 0.034 0.045..

0.030 0.056 0.075 0.100 0.068 0.090..

Using “SEND PLATE” you can send plate data to computer using either USB or SERIAL RS232.

Name: ABSORBANCE NORM – ABSORBANCE

Pri : 405 Sec : 630 BL N

D/M D/M D/M D/M D/M D/M

1 2 3 4 5 6

0.020 0.002 0.010 0.035 0.056 0.001

0.030 0.056 0.075 0.100 0.068 0.090

D/M D/M D/M D/M D/M D/M

7 8 9 10 11 12

0.020 0.002 0.010 0.0.35 0.056 0.001

0.030 0.056 0.075 0.100 0.068 0.090

ESC PRINTMATRIX SEND PLATE LOAD NEXT

The entire saved tests are available on first screen; tests can be selected from this screen also.

ABCDEFGHIJKLM

Select Test MENU

1 HBSAG SEL

PRNT

2 TSH NXT

PREV

3.ABSORBANCE DEL

MAIN MENU

1 Test Operations ESC

2 Technical Diagnosis

3 Utilities

4 View Plate

5 Serial Communication

TEST OPERATIONS

1 Select Test By Name ESC

2 Select Test by Mode

3 Select Test by Number

4 List Tests

5 Add New Tests

Select the test by “SEL” option.

Below screen will appear on the display after selecting the Run display.

The instrument will ask “Is Plate Loaded? YES / NO” Press Yes. Instrument will read the

absorbances using mechanical plate movement. After completion of reading it will ask:

NORM – ABSORBANCE

Name : ABSORBANCE

BL N ESC

Pri : 405 Sec : 630 EDIT

NORM RUN

MODE

DIFF PRINT

Name: ABSORBANCE NORM – ABSORBANCE

Pri : 405 Sec : 630 BL N

ABS ABS ABS ABS ABS ABS

1 2 3 4 5 6

ABS ABS ABS ABS ABS ABS

7 8 9 10 11 12

YES

Is Plate Loaded

NO

Name: ABSORBANCE NORM – ABSORBANCE

Pri : 405 Sec : 630 BL N

ABS ABS ABS ABS ABS ABS

1 2 3 4 5 6

ABS ABS ABS ABS ABS ABS

7 8 9 10 11 12

YES

Is Plate Removed

NO

Details of absorbance of all well in table format for each strip will display on the screen as shown

below.

In this operation if you keep BLANK

YES

The first well of first strip will be

considered as blank and instrument will

give the absorbance of remaining all well

with blank subtraction from original

absorbance.

The “PRINT MATRIX” will print the

details of entire plate with well

identification and absorbance of that

well, like…

A

W1 W2 W3 W4 W5 W6

0.125 0.250 0.350 0.450 0.550 o.650..

Using “SEND PLATE” plate data can be

sent to computer using either USB or

SERIAL RS232.

“LOAD NEXT” uses to get the

absorbance of next plate.

Name: ABSORBANCE NORM – ABSORBANCE

Pri : 405 Sec : 630 BL N

ABS ABS ABS ABS ABS ABS

1 2 3 4 5 6

0.125 0.250 0.350 0.450 0.550 0.650

-- --

--

--

--

0.125 0.250 0.350 0.450 0.550 0.650

ABS ABS ABS ABS ABS ABS

7 8 9 10 11 12

0.125 0.250 0.350 0.450 0.550 0.650

Differential Absorbance Mode: In differential absorbance mode the instrument will give the

absorbance difference each set of “ODD” number strip and EVEN number strip.

It is showing the absorbances only for

even number strips. This absorbance

values are actually differences between

each well of strip 1 and well of strip

2.You can get the difference between set

of 1-2, 3-4, 5-6, 7-8, 9-10 and 11-12. It is

not possible to change this combination.

In “PRINT MATRIX”, you will get the

print of entire plate data with actual

absorbance of each well and difference of

absorbance between ODD well and

EVEN well, like

A

W1 W2 W3 W4 W5

W6 0.200 0.100 1.200 0.300

2.600 0.900..

0.000 0.100 0.000 0.900 0.000

1.700..

B

W1 W2 W3 W4 W5

W6 0.300 0.100 1.200 0.200 2.600

0.800..

0.000 0.200 0.000 1.000 0.000

1.800..

Name: ABSORBANCE NORM – ABSORBANCE

Pri : 405 Sec : 630 BL N

ABS ABS ABS ABS ABS ABS

1 2 3 4 5 6

0.100 0.900 1.700

0.200 1.000 1.800

0.300 1.100 1.900

0.400 1.200 2.000

0.500 1.300 2.100

0.600 1.400 2.200

0.700 1.500 2.300

0.800 1.600 2.400

ABS ABS ABS ABS ABS ABS

7 8 9 10 11 12

0.100 0.900 1.700

0.200 1.000 1.800

0.300 1.100 1.900

0.400 1.200 2.000

0.500 1.300 2.100

0.600 1.400 2.200

0.700 1.500 2.300

0.800 1.600 2.400

ESC PRINTMATRIX SEND PLATE LOAD NEXT

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No Factor QC QCVal Mes. Rem SAVE

BL 1 Y 0.100

NC 3 1.000 Y 0.150

PC 2 1.000 Y 0.500

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.200

CUTABS: (0.45 * NC) + (0.35 * PC) + 0.10

QC1 > Y Interpretation :

QC2 N Greyzone % (N)

QC3 N Cut off Index (Y)

QC4 N Range Pos : 0.000

Neg : 0.000

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No. of Samples : 0 RUN

DUP N SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

B

NC

NC

NC

PC

PC

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No. of Samples : 10 RUN

DUP N SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

B S3

NC S4

NC S5

NC S6

PC S7

PC S8

S1 S9

S2 S10

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No. of Samples : 10 RUN

DUP N SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

B S3

NC S4

NC S5

NC S6

PC S7

PC S8

S1 S9

S2 S10

YES

Is Plate Loaded

NO

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No. of Samples : 10 RUN

DUP N SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

B S3

NC S4

NC S5

NC S6

PC S7

PC S8

S1 S9

S2 S10

YES

Is Plate Removed?

NO

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No

Factor QC QCVal Mes. Rem ACCEPT

BL 1 Y 0.100 0.050 TEST NC

3 1.000 Y 0.150 0.098

PC 2 0.100 Y 0.500 2.658 PRINT

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.200 CUTABS: 0.563 Display

VALUE:

QC1 2.560 QC2

QC3 QC4 LOAD

NEXT

Range % 10.00

absorbance. Next time it is possible to run the same test without loading controls. This means that

the previous stored values of controls can be used

All the details are in table format, as per row and column shown on the screen. You obtain the print

of the same format by selecting option ‘PRINT RESULTS’. To check the results of next samples,

choose option ‘NXT’, and than print it in format as shown on the screen by option ‘Print Results’

You can obtain the print in matrix format, by selecting option ‘PRINT MATRIX’.

In matrix form you will get the print as per your tray/plate for all the wells. Instrument prints seven

different parameters in print matrix for a single well.

ROW IDENTIFICATION A, B, C, D…

WELL NUMBER W1, W2, W3, W4…

WELL ID OR SAMPLE NUMBER BL, NC, PC, OR S1, S2, S3,

PATIENT IDENTIFICATION RAMESH, VIKAS, etc.

WELL ABSORBANCE 0.050, 0.098, 0.085, …

SAMPLE CONCENTRATION 0.738, 0.689, 2.578,…

INTERPRETATION / REMARKS POS or NEG or EQ

Name: HBSAG POS- Cut Off ESC

Pri: 450 Sec: 630 BL 1

Range PERCENTAGE % 10.00 NXT

Pos:0.330

CUT ABS= 0.300 Neg:0.270 PREV

NO SAMP ABS AVG CONC REM PID

A1 B 0.050 0.050

B1 NC

-F1 S1 0.200 0.200 0.254 NEG HIV-1

G1 S2 1.854 1.854 7.245 POS HIV-2

--

PRINT SAVE PRINT PLATE

RESULTS RESULTS MATRIX OPT.

If any individual control behaves incorrectly and it’s absorbance is not satisfying the QC check

value of that control, the following message appears: “Invalid Assay” and a remark “HI” or “LO”

for that control. OR if the QC1, QC2, QC3 and QC4, which has another QC condition of controls

(like some reagent manual gives condition PC-NC > 0.2 or NC/PC > 0.5) the following message

appears: “Invalid Assay” and a remark either ‘HI’ or ‘LO’ for that QC condition.

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No

Factor QC QCVal Meas. Rem ACCEPT

BL 1 Y 0.100 0.105 HI TEST

NC 3 1.000 Y 0.150 0.200 HI

PC 2 0.100 Y 0.500 0.456 LO PRINT

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.200 CUTABS: 0.563 Display

VALUE:

QC1 2.560 HI QC2

(NOTE: The absorbance

of controls should not go

above 3.50. If it is > than

3.50, it will show the

remark ‘HI’ for that

control. In such a case

edit the control

absorbance.)

In such case, delete an individual control, so that the average of remaining controls will satisfy the

QC condition of that control as per reagent manual.

To select any control for deleting, you select touch zone shown in the above screen.

When you select any particular control for deleting (suppose you have selected Negative Control

(NC), ‘HI’ or ‘LO’ remark appear on the screen.

“Select EDIT / DEL key to proceed”. To delete the control select ‘DEL’. After deleting that

control, the absorbance of that control will become zero and the average of controls will also get

changed. Select ‘ESC’ key to go back.

When you go back, you will get the edited value for average of control with no remark for any

control and no “Invalid Assay” message.

Make the blank absorbance valid in case the absorbance doesn’t meet the QC conditions.

If necessary valid the control absorbances because the absorbance of the blank affect the

absorbance of all controls and samples.

It is recommended that, for a test with single blank and single control, if absorbance doesn’t satisfy

QC conditions or if all controls of any single type (means all NC controls or all PC controls)

doesn’t meet the QC, the test will become totally invalid. It is not possible to make the VALID

TEST and new controls have to be loaded.

NC VALUE : 0.200

ESC

NC1 0.150 DEL

NC2 0.350 HI

NC3 0.200 HI

NC VALUE : 0.200

ESC

NC1 0.150 DEL

NC2 0.350 HI

NC3 0.200 HI

Name: TSH MULTISTANDARD

Pri: 450 Sec: 630 ESC

No. of samples : 0 RUN

DUP N SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

B

C1

C2

C3

C4

C5

C6

C7

HI CO: 0

COL

ROW

Name: TSH MULTISTANDARD

Pri: 450 Sec: 630 BL Y 0.000 CAL 7 ESC

DUP N DUP N

EDIT

Graph : LINEAR

Y vs X : ABS vs CONC RUN

HI CO : N High>25.03 PRINT

Low<10.54

LO CO: N High>16.61

Low<5.45

Interpretation: Y

Range High> 15.25

Low< 9.46

To change values press EDIT

Numeric Value

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

HI CO : 2

ROW COL

2

3

Please Enter Col Entry: 1

Numeric Value:

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

HI CO : 2

ROW COL

A 2

B 3

Please Enter Row Entry: 1

Name: TSH MULTISTANDARD

Pri: 450 Sec: 630 ESC

No. of samples : 0 RUN

DUP N SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

B HC

C1 HC

C2

C3

C4

C5

C6

C7

HI CO: 2

COL

ROW

Name: TSH MULTISTANDARD

Pri: 450 Sec: 630 ESC

No. of samples : 12 RUN

DUP N SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

B HC S8

C1 S1 HC

C2 S2 S9

C3 S3 S10

C4 S4 S11

C5 S5 S12

C6 S6

C7 S7

The tray will come out. The following message appears: “Is Plate loaded YES / NO”.

The tray has to be loaded in a direction that calibrators should go in first and press YES.

The instrument will read the absorbance of calibrators and samples.

“Is plate removed? YES / NO”. Remove the tray and press YES.

The Tray holder will go inside and on the screen all details of calibrators, samples and controls are

shown.

All the details as per row and column are shown on the screen. The printout of the same format can

be obtained by selecting option ‘PRINT RESULTS’. To check the results of next samples, choose

option ‘NXT’and print the format shown on screen by option ‘Print Results’.

You can get the print in matrix format, by selecting option ‘PRINT MATRIX’.

In matrix form you will get the print as per your tray/plate for all the wells. Instrument will print

seven different parameters in print matrix for a single well as follows:

Name: TSH MULTI STANDARD ESC

Pri: 450 Sec: 630 BL Y CAL 7

Range High > 16.61 Low < 10.30 NXT

HI CO : High > Low < 0.000

LO CO : High > Low <

PREV

NO SAMP ABS AVG CONC REM PID

A1 B 0.050 0.050 SAVE

B1 C1 0.070 0.070 0.000 RESULT

C1 C2 0.257 0.257 2.500

--

--

--

F1 S1 0.200 0.200 2.100 NEG TSH-1

G1 S2 1.854 1.854 9.845 POS TSH-2

LOAD PRINT PLATE VIEW ACCEPT PRINT

NXT MATRIX OPT. GRAPH: TEST RESULTS

ROW IDENTIFICATION A, B, C, D…

WELL NUMBER W1, W2, W3, W4…

WELL ID OR SAMPLE NUMBER B, C1, C2, C3, OR S1, S2, S3,

PATIENT IDENTIFICATION RAMESH, VIKAS, etc.

WELL ABSORBANCE 0.050, 0.098, 0.085, …

SAMPLE CONCENTRATION 0.738, 0.689, 2.578…

INTERPRETATION / REMARKS POS, NEG, EQ,

Origin at : X =0.000 , y = 0.000

X / div = 2.500 , y / div = 0.150 ESC

3.000

EDIT

1.000

0.000 10.00 20.00 40.00

Select ‘ACCEPT TEST’ option to save this test with the details of calibrator absorbance. It is

possible to run the same test without loading calibrators. The previous graph can be used for new

samples.

(* NOTE: If you do not want to run the calibrators each time, please select “ACCEPT TEST”

option to save graph, whenever you run the test with calibrators.)

In Multi standard, the absorbance of calibrators should be in increasing or decreasing from one

calibrator to the next calibrator. If any calibrator/s behave(s) incorrectly you will obtain a message

“Invalid Assay” at bottom side of screen.

In such a case “EDIT” option is available on the GRAPH screen. If you select EDIT,

the following screen will appear:

Name: TSH MULTI STANDARD ESC

Pri: 450 Sec: 630 BL Y CAL 7

Range High > 16.61 Low < 10.30 NXT

HI CO : High > Low < 0.000

LO CO : High > Low <

PREV

NO SAMP ABS AVG CONC REM PID

A1 B 0.050 0.050 SAVE

B1 C1 0.070 0.070 0.000 RESULT

C1 C2 0.257 0.257 2.500

LOAD PRINT PLATE VIEW ACCEPT PRINT

NXT MATRIX OPT. GRAPH: TEST RESULTS

CAL 1 2.562 ESC

CAL 2 1.865

CAL 3 2.050

CAL 4 1.054

CAL 5 0.648

CAL 6 0.356

CAL 7 0.045

CAL 1 2.562 ESC

CAL 2 1.865

CAL 3 2.050

CAL 4 1.054

CAL 5 0.648

CAL 6 0.356

CAL 7 0.045

Numeric Value:

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

You can easily select any particular calibrator by touching that touch zone and edit the absorbance

of that. After editing the calibrator’s absorbance select “ESC”. A message will appear: “PLEASE

WAIT…” and the modified graph will appear on the screen.

(*NOTE: SAME PROCEDURE CAN BE USED TO CREATE AND RUN THE TEST IN ALL

OTHER MODES, LIKE SINGLE STANDARD, % ABSORBANCE AND UPTAKE.)

“ACCEPT TEST” option is used to store the data of controls or calibrators (refer to chapter 9.2,

9.3). After running any test first time with controls or calibrators, you select option “Accept Test”

to save the data of controls or calibrators, so that next time when you want to load the same test,

there is no need to load controls or calibrators in ELISA plate. You can use previously stored data.

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No

Factor QC QCVal Mes. Rem ACCEPT

BL 1 Y 0.100 0.050 TEST NC

3 1.000 Y 0.150 0.098

PC 2 0.100 Y 0.500 2.658 PRINT

LC 0 1.000 N

CC 0 1.000 N

CUTFAC: 0.200 CUTABS: 0.563 Display

VALUE:

QC1 2.560 QC2

QC3 QC4 LOAD

NEXT

Range % 10.00

Name: TSH MULTI STANDARD ESC

Pri: 450 Sec: 630 BL Y CAL 7

Range High > 16.61 Low < 10.30 NXT

HI CO : High > Low < 0.000

LO CO : High > Low <

PREV

NO SAMP ABS AVG CONC REM PID

A1 B 0.050 0.050 SAVE

B1 C1 0.070 0.070 0.000 RESULT

C1 C2 0.257 0.257 2.500

F1 S1 0.200 0.200 2.100 NEG TSH-1

G1 S2 1.854 1.854 9.845 POS TSH-2

LOAD PRINT PLATE VIEW ACCEPT PRINT

NXT MATRIX OPT. GRAPH TEST RESULTS

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No. of Samples : 0 RUN

DUP N SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No. of Samples : 0 RUN

DUP N SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

This time, the blank and

controls will not be loaded

directly and you will get the

following question: “Load

Blank? YES / NO” and

“Load Controls? YES / NO”.

If you are running both, select

‘YES’, and if you want to use

earlier stored data / graph of

controls select ‘NO’, so that

you can run only samples to get

their results without loading

control and calibrators.

If you want to load only

‘Blank’,select “Load Blank?

YES” and

“Load Controls? NO”.

While loading the samples you can load a single sample in

single well or a single sample in adjacent two well and finally

the instrument will take the average of it as known as Sample

Duplicate.

The instrument will load the controls / calibrators

automatically in any new test or not accepted test. You have to

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No. of Samples : 0 RUN

DUP N SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

In Calibrator Mode, after asking

“Load Blank? Yes / No” you will

get the following question “Load

Calibrators? Yes / No”. If you are

loading new calibrators and want

the results of samples as per new

graph select “Load Calibrators?

YES”.

If you are not loading new

calibrators and want to use

previously stored graph select

“Load Calibrators? No”.

If you want to load only ‘Blank’,

select “Load Blank? YES” and

“Load Calibrators? NO”.

While loading the samples you can load a single sample in single well or a single sample in

adjacent two well and finally instrument takes the average of it; this is known as Sample Duplicate.

The instrument will load the controls / calibrators automatically in any new test or not accepted

test.

You have to provide the number of samples. If you are loading single samples keep “DUP”- No.

Select “No. of Samples:” to enter total samples.

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No. of Samples : 0

RUN

DUP N SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

B

NC

NC

NC

PC

PC

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No. of Samples : 0

RUN

DUP N SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

B

NC

NC

NC

PC

PC

Numeric Value:

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630

ESC

No. of Smples : 10

RUN

DUP N SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

B S3

NC S4

NC S5

NC S6

PC S7

PC S8

S1 S9

S2 S10

If you want to load duplicate samples select “DUP” key, and on the screen ‘N’ change to ‘Y’.

After this, enter number of samples by selecting “No. of Samples”.

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630

ESC

No. of Samples : 0

RUN

DUP Y SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

B

NC

NC

NC

PC

PC

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630

ESC

No. of Samples : 10

RUN

DUP Y SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

B

NC

NC

NC

PC

PC

Numeric Value:

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

(* Same way you load the samples and Sample Duplicates in all other modes)

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No. of Samples : 10 RUN

DUP Y SIMUL PID

1 2 3 4 5 6 7 8 9 10 11 12

B S2 S6 S10

NC S2 S6 S10

NC S3 S7

NC S3 S7

PC S4 S8

PC S4 S8

S1 S5 S9

S1 S5 S9

After entering number of samples either single or duplicate, select “PID”.

One by one you can enter name of each patient. The character length of PID is only 7 characters.

Go to the next and previous screen with “NEXT” and “PREV”

Name: HBSAG POS- Cut Off

Pri: 450 Sec: 630 ESC

No. of Smples : 10 RUN

1 2 3 4 5 6 7 8 9 10 11 12

B S3

NC S4

NC S5

NC S6

PC S7

PC S8

S1 S9

S2 S10

Sample PID ESC

1 KIRAN

2 ABDUL

3 MR. JAME

4 KRISHNA

5

6

7 NEXT

8

9 PREV

10

SEQL

This one by one entry will take more time, therefore one more option to enter patient Ids is

provided. This option is “SEQL”sequential entry.

If you select “PID” option for patient ID entry, the following question appears on the screen:

“Delete Previous PIDs, Yes / No”. If you select NO, it will directly load the previously entered

PIDs for sample and if you say YES, it will delete the previous PIDs.

After selecting ‘NO’, select “SEQL” option to enter different sequential PIDs. And follow the steps

as follows:

Enter Alphabets

HIVA

B C D E F G H

I J K L M N O P ENTER

Q R S T U V W X CLEAR

Y Z , - + _ 1 2

3 4 5 6 7 8 9 0

Enter Initial Sample number

Numeric Value: 01

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

Enter last sample number

Numeric Value: 01

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

Select ‘SEQL’, Enter alphabet ‘HIV’. Enter initial well number ‘1’ and the last well number ‘30’,

after this enter initial PID number ‘1’. It will load PIDs for first 30 samples from HIV 1 to HIV 30.

Enter initial PID number

Numeric Value: 01

1 2 3 4 5 6 ENTER

7 8 9 0 . CLEAR

Sample PID ESC

1 HIV-01

2 HIV-02

3 HIV-03

4 HIV-04

5 HIV-05

6 HIV-06

7 HIV-07 NEXT

8 HIV-08

9 HIV-09 PREV

10 HIV-10

SEQL

To enter next sequence again select ‘SEQL’, enter alphabet ‘HIV’. Enter initial well number ‘31’

and last well number ‘55’. After this enter initial PID number ‘51’. It will load PIDs for next 25

samples from HIV 51 to HIV 75.

To enter next sequence again select ‘SEQL’, enter alphabet ‘HIV’. Enter initial well number ‘56’

and last well number ‘70’. After this enter initial PID number ‘91’. It will load PIDs for next 15

samples from HIV 91 to HIV 105.

In this way you can select any sequence for any well locations.

(*NOTE: The entered PID gets stored in instrument memory, until you delete it by using option,

“Delete previous PIDs? Yes / NO.”)

In all modes of operation, there are two options provided to store results.

If “PLATE OPT.”is selected, you will obtain two options.

“SAVE PLATE DATA”,

which will save the entire plate information along with the control/calibrator information.

(maximum TEN plates data can be saved).

“SEND PLATE DATA” is used to send the data from

instrument to computer through USB or serial RS232

connection.

If “SAVE RESULT” option is selected the information of samples only except controls/calibrators,

with test name, patient ID and date will be saved.

Name: HBSAG POS- Cut Off ESC

Pri: 450 Sec: 630 BL 1

Range PERCENTAGE % 10.00 NXT

NO SAMP ABS AVG CONC REM PID PREV

A1 B 0.050 0.050

B1 NC

Name: TSH MULTI STANDARD ESC

Pri: 450 Sec: 630 BL Y CAL 7

Range High > 16.61 Low < 10.30 NXT

HI CO : High > Low < 0.000

LO CO : High > Low <

PREV

NO SAMP ABS AVG CONC REM PID

A1 B 0.050 0.050 SAVE

B1 C1 0.070 0.070 0.000 RESULT

C1 C2 0.257 0.257 2.500

--

1. SAVE PLATE DATA

ESC

2. SEND PLATE DATA

The stored plate or stored results appear on the screen by selecting option “View Plate” in “MAIN

MENU”.

Two options appears on the screen by selecting “View Plate” option

1) The details of stored plate (last 10 plates) with date, test name and mode of operation in

descending order from last to first will appear on the screen by selecting. “PLATE RESULTS”

Any test can be selected. The complete information will appear on the screen.

“Print Matrix” will print the details of entire plate in matrix format as explain earlier.

“Print” will print only the data displayed on screen.

“Send data” can be used to send the data from instrument to computer through USB or serial

RS232 connection.

2.) “PATIENT RESULTS” will show the details of all samples, saved in memory using option

“SAVE RESULTS” in all different modes, as explain earlier. This option will display the data in

table format as shown below. Test name, Mode of test operation, result of sample, PID and Remark

will appear on the screen. The instrument has a memory to store 2500 sample results.

This option doesn’t show the information of controls or calibrators.

MAIN MENU

1 Test Operations ESC

2 Technical Diagnosis

3 Utilities

4 View Plate

5 Serial Communication

1. PLATE RESULTS

ESC

2. PATIENT RESULTS

“PRINT” is used to print the details as available on the screen.

“NEXT” and “PREV” option are used to go to next page of sample details and to go back to

previous page.

(*NOTE: When there is not enough memory the following message appears:

“Memory Left for samples- First Samples Deleted”)

The instrument automatically deletes a number of samples that are stored in the beginning and than

save the new samples at that location.

HBSAG 02/01/08

CUT ABS= 0.300

NO SAMP ABS AVG RES REM PID

01 / 01 / 08

Test Mode Result PID Remarks

In utilities there are seven different functions

Set current Date and Time by

Selecting DD, MM, YY and

Hrs, Min, Sec.

MAIN MENU

1 Test Operations ESC

2 Technical Diagnosis

3 Utilities

4 View Plate

5 Serial Communication

Date – DD / MM / YY ESC

01 / 01 / 08

Time – Hrs : Min : Sec

12 : 10 : 10

15.3. Clinic Name Entry.

Type your Clinic Name and select “ENTER”. The character length is maximum 19 characters.

A. D. TOUCH

A B C D E F G H

I J K L M N O P ENTER

Q R S T U V W X CLR

Y Z , - + _ 1 2

3 4 5 6 7 8 9 0

The total shake time can be entered in seconds.

Regarding the “Shake Speed”, there are 10 types of speeds. You can choose any speed by entering

number from 1 to 10. The shaking speed increases as the number increases.

After entering shake time and shake speed, select Start option. When “Start” is selected, the tray

will come out and on the screen you will obtain a message “Is plate loaded? Yes / No”.

After selecting YES the tray will go inside and instrument will do the shaking of plate for the given

time period with selected speed.

When the shaking is finished, the play will come out of the tray and display a message

“Is plate removed? Yes / No “Remove the tray and press Yes to end the process.

Shake Time in 0 sec ESC

Shake Speed 0

Start

Stop

Numeric Value: 01

1 2 3 4 5 6 ENTR

7 8 9 0 . CLR

This setting is very important, whenever you want to transfer data from instrument to computer.

You can transfer data either using USB or by Serial RS232.

We provide one application which support to this communication between instrument and

computer. This application receives data either by USB setting or by Serial setting.

Technical Diagnosis is present in “Main Menu”.

This option is used to clear entire saved test records and entire saved patient results along with

saved plates. The “Test Records” are the different kind of programmed test that are made in

different modes of operation and saved in instrument program memory.

When option “Clear Memory” is selected the instrument will first ask “Clear Results YES / NO”.

If “YES” is selected it will only clear saved patient results and saved plates stored in the memory,

and shows message “Clearing Memory …”.

This option does not clear the programmed test saved in instrument memory.

After clearing the memory or if option “NO” is selected the next question appears.

MAIN MENU

1 Test Operations ESC

2 Technical Diagnosis

3 Utilities

4 View Plate

5 Serial Communication

YES

Clear Results?

NO

This option is used to check the light gain of filters used in the instrument. There are 8- channels

used of same type. This option shows the gain of each channel filter in table format. In the

instrument the range of amplitude is from 600.00 to 2450.00. If the amplitude goes below 600.00

for any channel, the message “Amp –Low” will appear on the screen for that particular channel

if the amplitude goes more than 2490.00, than the over range saturation voltage figure of 2500.00

will be passed.

The gains of filters are set within a required range. When you select this option, the lamps will turn

ON, tray holder plate will comes out and the instrument will start showing amplitudes (gains) of all

filters one by one.

Filter: 405 ESC

CH1 CH2 CH3 CH4

1800.0 2200.0 1900.0 2300.0

1800.0 2200.0 1900.0 2300.0

CH1 CH2 CH3 CH4

1950.0 2100.0 2240.0 1860.0

1950.0 2100.0 2240.0 1860.0

It is used to check the printing of thermal printer. Is it printing perfectly or not?

1) Printer disabled

Disable Printer YES / NO?

You will get this message, if paper is not loaded properly or lever is not

at correct position. Check all possibilities. Also check ON LINE and

FEED LED glowing or not and when you switch ‘ON’ the instrument

check paper is moving forward or not. If LEDs are not glowing and

paper is not moving forward contact service engineer.

2) “Check Light Path!!! CH x”

Where ‘x’ is any channel

number

X = 1/2/3/4/5/6/7/8

You can get this message in any mode of operation. Before reading

absorbance, instrument is checking the filter reference voltages of all 8

channels. If filter voltage of any channel is less than minimum required

voltage, it will display a message “Check Light Path!!! CH x”. Here ‘x’

is channel numbers having reference voltage less than minimum

required.

For ex. If reference voltage of channel 3 is less the message will be

displayed “Check Light Path!!! CH 3”. It will indicate all channel

numbers having less reference voltage, like

“Check Light Path!!! CH 1 3 6 ”

In such a case, there is a possibility of filter gain of that channel is

reduced or intensity of lamp of that channel has become poor, so contact

factory engineer.

3) Invalid Assay

If in the Cut Off and Multi standard mode the controls and standards are

not ok then this error message will appear.

4) Memory Full

If Number of saved tests exceeds the memory limit of 250 tests then

delete the unwanted tests and save the test.

Computer

Do you want to continue?

Using data receiving application on computer,

1) If you have not connected the data cable (USB or Serial) correctly,

you will get this message.

2) If you have selected ‘Communication Setting’ (ref. 14.5), USB on

instrument, and you have selected a ‘Serial’ communication on

computer data receiving application, you will get this message. In such

a case select ‘USB’ communication, on computer and select ‘YES’ key

to transfer data.

To avoid this message, connect proper data cable, select the same

communication medium (USB or Serial), on computer data receiving

application, which you have selected on instrument ‘Communication

Setting’.

6) Filter Movement Error!!!

Instrument will display this message, if there is problem with

MOC sensor PCB or with filter tray stepper motor. The MOC

sensors are used to detect the position of filter tray. These two

sensors are mounted on bottom of the mechanism. One detects the

home position and second detects the each filter position of filter

tray. In such a condition we need to check the connection to these

sensor and also the connection of stepper motor, which is used to

move filter tray.

7) Plate Movement Error!!!

Instrument will display this message, if there is problem with

MOC sensor PCB or with plate carrier stepper motor. The MOC

sensors are used to detect the position of plate carrier. These two

sensors are mounted on top of the mechanism. One detects the

home position and second detects the each strip position of plate

carrier. In such a condition we need to check the connection to

these sensor and also the connection of stepper motor, which is

used to move plate carrier.

If the instrument has to be shipped after being exposed to potential hazard material, it should be

decontaminated. The following procedure outlines the method of decontaminating the instrument

before packaging and shipment.

Decontamination minimizes the risk to all who come in contact with the instrument during

shipping, handling, and servicing.

Any laboratory instrument that has been used for clinical analysis is considered as a biohazard and

should be decontaminated prior to handling. Intact surface is generally considered as an effective

barrier against infectious organisms. However, small abbreviations may not be always being

visible. Gloves must be used incase the instrument is not decontaminated.

Mucous membranes are considered prime entry routes for infectious agents. Wear eye protection

and a surgical mask when there is a possibility of aerosols.

Eating and drinking while decontaminating instruments is not advisable.

A solution of 5% Sodium Hypo Chlorite (NaOCL) solution (Bleach) is used. Commercial bleach is

5% NaOCL; household bleach is 3% NaOCL. When using commercial bleach, use a 10:1 mixture;

if using household bleach, a 6:1 mixture is required. This is a caustic solution. It is important to

wear gloves and eye protection when handling it.

Wipe down the carrier and all exposed surfaces of the unit with the bleach solution. Remove the

top shroud of the instrument and wipe down the top surface of the instrument base, as well as the

inside of the top shroud.

Reassemble the unit and discard the used gloves and towels.

Please complete all information requests on this form prior to returning the instrument to the

manufacturer or your local distributor for servicing, repairs or return. Thank you for your cooperation.